detector (the ACQUITY UPLC Photodiode Array (P.D.A.) Detector) (Waters Corp, Milford, MA, USA). A phenolic compound analysis was performed using an Acquity H class UPLC system equipped with an Acquity P.D.A. Next, samples were dissolved in 1 mL methanol (Sigma-Aldrich, Inc., St. An automated N1 system was used, which specializes in the rapid concentration of samples by PERLAN Technologies. The produced ether extracts were continuously transferred to 8 mL vials, after which they were evaporated to dryness in a stream of nitrogen. After being cooled in water with ice, the samples were extracted with diethyl ether (2 × 2 mL for approx. The tightly sealed test tubes were heated in a water bath at 95 ☌ for 30 min. For this purpose, the aqueous phase was supplemented with 3 mL 6 M aqueous hydrochloric acid solution. obliquus sclerotia indicate their broad biological activity and potential properties, including hypoglycemic, antiviral, antimutagenic, anticancer, and cytostatic characteristics, as well as strong antioxidative properties. The results of contemporary studies of the metabolites isolated from I. obliquus sclerotia (black, cracked, lumpy formations) from birch trunks are used for medicinal purposes. list, it is an edible mushroom in Canada and Russia (Journal of Laws No. In Poland, the mushroom is considered inedible, while according to the F.A.O.
obliquus occurs infrequently, and it is mainly found on birch and rarely on other deciduous trees therefore, it is under partial species protection. obliquus (Fr.) Pilát, which also causes white rot in wood. Īnother mushroom with healing properties is I. versicolor fruiting bodies, which are responsible for many therapeutic effects, including polysaccharides, protein-polysaccharide complexes, polyphenolic compounds, and terpenes. Mycological and chemical research has proven the presence of many compound groups in T. listed the mushroom as edible in China, Hong Kong, Laos, and Mexico. In Europe, it is considered an inedible mushroom however, the mushrooms list prepared for the F.A.O. The fruiting bodies can be found on the trunks or branches of dead shrubs and angiosperm and gymnosperm tree, which cause white rot in the wood. Another common saprobiont throughout Europe is T. Campb.) Verkley, Göker & Stielow, Penicillium citrinum Thom, and Pleurotus tuber-regium (Fr.) Singer.
fasciculare against other soil fungi and antibacterial activity were also found in the case of Paraphaeosphaeria minitans (W.A. A compound with an antibiotic effect against Staphylococcus aureus was isolated from its fruiting bodies. Poisonous fungi commonly found in the forest environment include H. obliquus) reported the highest content of tested compounds. A diversified content of bioactive compounds characterized the tested fungi, and the Chaga ( I. The ergosterol was quantified in the saprobiont fungi cultures. In addition, the observed concentration of naringenin, quercetin, and kaempferol above 21 mg/kg DM was found. High concentrations of flavonoids were observed in H. All isolates were tested for β-carotene, lutein, zeaxanthin, and astaxanthin. The antioxidant activity was from 2.5 to 3.5 times higher in the isolates of I. In the tested isolates, the presence of 13 phenolic acids has been observed, including p-coumaric, ferulic, chlorogenic, and sinapic acid, in high concentrations. Chemical methods (HPLC determination, ABTS + and FPA methods, and a saponification method by Acquity UPLC) were used to analyze active compounds. Karst., Inonotus obliquus (Fr.) Pilát, Kuehneromyces mutabilis (Schaeff.) Singer & AH Sm., Trametes versicolor (L.) Lloyd, Pleurotus ostreatus (Jacq.) P. The presented study aimed to determine the content of selected antioxidant active compounds of selected saprobionts commonly found in the European forests: Hypholoma fasciculare (Huds.) P. Wood rot fungi are an essential link in the forest ecosystem.
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